It was claimed earlier this year that the Dark Knight star was in talks to join a potential DC comics ensemble film, although the speculation was denied by writer David S Goyer.
Bale has no told Entertainment Weekly that he has no intention of reprising his role in any future projects.
"We were incredibly fortunate to get to make three [Batman films]," the 39-year-old explained. "That's enough. Let's not get greedy."
"Chris [Nolan] always said he wanted to make it one film at a time. And we ended up sitting there looking at each other, saying, "We're about to make the third'. We never really knew if we were going to get to be there, but if that was how it was going to be, this was where it should end as well."
On the role of the Caped Crusader, he added: "It's a torch that should be handed from one actor to another. So I enjoy looking forward to what somebody else will come up with."
Bale went on to say that he does not know anything about a Justice League movie, which has been heavily rumored following the box office success of Zack Snyder's Man of Steel.
"I have no information, no knowledge about anything," Bale insisted. '
"I've literally not had a conversation with a living soul. I understand that they may be making a Justice League movie, that's it."